Project & Microscope Requests

user steps

1. Register in the SLAC User Portal
2. Submit a Project Proposal (via User Portal)
3. Submit a Microscope Time Request (via User Portal)
4. Sign Access Agreement (external collaborators) and unix account request
5. Discuss final logistics with cryo-EM staff (2 weeks prior to scheduled microscope time)
6. Transport Samples to SLAC (1 week prior to scheduled microscope time)
7. Access cryo-EM data via SLAC's global file system

New Multi-Technique Proposals (Pilot)

A new Multi-Technique Proposal mechanism has been created for projects that require the use of two or more scientific techniques available at SSRL (both x-ray and electron based). The pilot proposal mechanism will initially cover Small Angle X-ray Scattering, Macromolecular Crystallography and Cryo-Electron Microscopy. A goal is to expand the mechanism in its permanent phase to include additional techniques available at LCLS and spectroscopy and imaging methods at SSRL.

The new pilot proposals will require a thorough justification for the requirement of using two or more techniques.  The proposals will be reviewed by an ad hoc Proposal Review Panel comprised of members from the SSRL SMB and Cryo-EM PRPs.  The new proposal template is now available and the first deadline for submission is July 1.

To get started:  Log into User Portal  -> Select SSRL as the Facility -> Choose Submit Structural Biology-related Multi-Technique Proposal from SSRL Proposals pulldown menu.

Contact Stanford-SLAC Cryo-EM

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Instructions for requesting Stanford-SLAC cryo-EM resources

1. Project Proposal

Experimental access to Stanford-SLAC Cryo-EM Facilities can be obtained by setting up a proposal through the User Portal. Stanford/SLAC Principal investigators and team members should register in the portal before proceeding with a project proposal. Once registration is complete contact the Cryo-EM User Office to set up the proposal and identify a lead contact.

2. Microscope Time Request

Requests for on-site or remote time to collect images from your samples must be submitted through a Microscope Time Request. Sample details and variations from the original project proposal are to be included in the Microscope Time Request. A call for requests is made on a quarterly basis. The management team will make allocation determinations after Microscope Time Requests have been reviewed for safety and hazardous material concerns by SLAC/Stanford staff.  Collaborators from external institutions are required to execute either a stand-alone Cryo-EM User Agreement or an addendum if there is an existing DOE User Agreement covering experiments at the SSRL and the LCLS. More information about access agreements can be found here.  Questions regarding Microscope Time Request submittal can be directed to the Cryo-EM User Office.

3. Sample Shipment to and from SLAC:  If applicable - users will ship the frozen grid to SLAC one week prior to the date of data collection. The sample and the dry shipper will be sent back to the users after one week of the data collection - please ensure a pre-paid return label is included with your shipment. We can archive the frozen grid during the period of the project. If the user desires to have the sample and its Dewar back, the user is responsible for all shipping costs. Shipping instructions

4. Remote Access: Secured remote access to operate the cryo-electron microscopes is available. Users who wish to make use of this must apply for a SLAC unix account and indicate remote data collection a part of the the Microscope Time Request. Further details here.

5. Data Transfer: As soon as your experiment has started, your experimental data will be made available on our central data storage. A SLAC unix account is required to access the data. Standard unix tools like scp and rsync are available, as is Globus. Further details here.

6. Data Storage and Analysis: SLAC will provide a short term (3 months) storage on disk and long term (3 years) on magnetic tape. On the fly data analysis to evaluate image quality is available. The more extensive computing resources required for 3-D reconstruction are available through a competitive application process.